Journal: Scientific Reports

Article Title: Inflammatory corpuscle AIM2 facilitates macrophage foam cell formation by inhibiting cholesterol efflux protein ABCA1

doi: 10.1038/s41598-024-61495-4

Figure Lengend Snippet: AIM2 is a negative regulator of ABCA1. ( a ) AIM2 and ABCA1 protein expression levels in THP-1 macrophages. ( b ) Correlation analysis of relative protein expression levels of AIM2 and ABCA1. n = 3, Comparison with NC group, **p < 0.01; Comparison with MC group, ##p < 0.01.

Article Snippet: Then the PVDF membranes were incubated with anti-ABCA1 (abclonal A22125, 1:1000), AIM2 Rabbit pAB (abclonal A3356, 1:1000) and GAPDH (abclonal A 19056, 1:1000) at 4 °C overnight.

Techniques: Expressing, Comparison

Journal: Frontiers in Pharmacology

Article Title: Investigation of the Lipid-Lowering Mechanisms and Active Ingredients of Danhe Granule on Hyperlipidemia Based on Systems Pharmacology

doi: 10.3389/fphar.2020.00528

Figure Lengend Snippet: The detail information of three pathways mainly involved in lipid metabolism.

Article Snippet: Anti-SREBP-1c, anti-liver X receptor alpha (LXRα), anti-cholesterol 7α-hydroxylase (CYP7A1), and anti-ATP binding cassette subfamily A member 1 (ABCA1) were purchased from ImmunoWay Biotechnology Company (Newark, DE, USA).

Techniques: Control

Journal: Frontiers in Pharmacology

Article Title: Investigation of the Lipid-Lowering Mechanisms and Active Ingredients of Danhe Granule on Hyperlipidemia Based on Systems Pharmacology

doi: 10.3389/fphar.2020.00528

Figure Lengend Snippet: Effect of DHG on mRNA expression of genes in hamster liver by RT-PCR. Values are mean ± SD, n = 5. (A) SREBP-1c mRNA. (B) FAS mRNA. (C) SREBP-2 mRNA. (D) LDLR mRNA. (E) PPARα mRNA. (F) LXRα mRNA. (G) CYP7A1 mRNA. (H) ABCA1 mRNA. RT-PCR, real-time polymerase chain reaction; SREBP-1c, sterol regulatory element-binding protein-1c; FAS, fatty acid synthase; SREBP-2, sterol regulatory element binding protein 2; LDLR, low density lipoprotein receptor; PPARα, peroxisome proliferation-activated receptor alpha; LXRα, liver X receptor alpha; CYP7A1, cholesterol 7α-hydroxylase; ABCA1, ATP binding cassette subfamily A member 1. * p < 0.05 vs NC; ** p < 0.01 vs NC; # p < 0.05 vs HFD; ## p < 0.01 vs HFD.

Article Snippet: Anti-SREBP-1c, anti-liver X receptor alpha (LXRα), anti-cholesterol 7α-hydroxylase (CYP7A1), and anti-ATP binding cassette subfamily A member 1 (ABCA1) were purchased from ImmunoWay Biotechnology Company (Newark, DE, USA).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Binding Assay

Journal: Frontiers in Pharmacology

Article Title: Investigation of the Lipid-Lowering Mechanisms and Active Ingredients of Danhe Granule on Hyperlipidemia Based on Systems Pharmacology

doi: 10.3389/fphar.2020.00528

Figure Lengend Snippet: Effect of DHG on protein expression of targets in hamster liver by Western blot. Values are mean ± SD, n = 3. (A) SREBP-1c, FAS. (B) SREBP-2, LDLR. (C) PPARα, LXRα, CYP7A1. (D) ABCA1. * p < 0.05, ** p < 0.01 vs NC group; # p < 0.05, ## p < 0.01 vs HFD group. The expression levels were normalized by GAPDH.

Article Snippet: Anti-SREBP-1c, anti-liver X receptor alpha (LXRα), anti-cholesterol 7α-hydroxylase (CYP7A1), and anti-ATP binding cassette subfamily A member 1 (ABCA1) were purchased from ImmunoWay Biotechnology Company (Newark, DE, USA).

Techniques: Expressing, Western Blot

Journal: International Journal of Oral Science

Article Title: Antisense oligonucleotide targeting TARDBP-EGFR splicing axis inhibits progression of oral squamous cell carcinoma through ABCA1-regulated cholesterol efflux

doi: 10.1038/s41368-025-00402-7

Figure Lengend Snippet: EGFR isoforms differentially inhibit cell proliferation by facilitating ABCA1 activation. a Western blotting analysis of the effect of EGFR isoform overexpression on the phosphorylation of EGFR. b , c Immunohistochemistry (IHC) staining of pEGFR and ABCA1. Scale bars, 100 μm (left), 25 μm (right). d The protein expression of ABCA1 in HN6 and CAL27 cells overexpressing EGFR-001 or EGFR-004 was detected by western blotting. e Co-immunoprecipitation analysis of the interaction between ABCA1 and EGFR in HN6 and CAL27 cells transfected with control, EGFR-001 , or EGFR-004 plasmids

Article Snippet: The lysates were then incubated overnight at 4 °C with either an anti-ABCA1 antibody (DF8233, Affinity Biosciences) or IgG (30000-0-AP, Proteintech) with constant rotation.

Techniques: Activation Assay, Western Blot, Over Expression, Phospho-proteomics, Immunohistochemistry, Expressing, Immunoprecipitation, Transfection, Control

Journal: International Journal of Oral Science

Article Title: Antisense oligonucleotide targeting TARDBP-EGFR splicing axis inhibits progression of oral squamous cell carcinoma through ABCA1-regulated cholesterol efflux

doi: 10.1038/s41368-025-00402-7

Figure Lengend Snippet: Ubiquitination levels of different EGFR isoforms. a Western blotting showing the ABCA1 protein in CAL27 and HN6 cells transfected with control, EGFR-001 , or EGFR-004 plasmids and treated with CHX (20 μg/mL) for the indicated times. b Western blotting of ABCA1 in HN6 and CAL27 cells transfected with EGFR-001 , EGFR-004 or control vectors, followed by treatment with 20 μmol/L MG132 for 6 h. c Ubiquitination level of ABCA1 protein in HN6 and CAL27 cells treated with MG132 and overexpressed with EGFR-001 , EGFR-004 were detected by western blotting

Article Snippet: The lysates were then incubated overnight at 4 °C with either an anti-ABCA1 antibody (DF8233, Affinity Biosciences) or IgG (30000-0-AP, Proteintech) with constant rotation.

Techniques: Ubiquitin Proteomics, Western Blot, Transfection, Control

Journal: International Journal of Oral Science

Article Title: Antisense oligonucleotide targeting TARDBP-EGFR splicing axis inhibits progression of oral squamous cell carcinoma through ABCA1-regulated cholesterol efflux

doi: 10.1038/s41368-025-00402-7

Figure Lengend Snippet: ABCA1 modulates membrane fluidity and Vimentin-mediated EMT in OSCC cells via cholesterol efflux. a Relative membrane cholesterol levels after overexpression of different EGFR isoforms. b Filipin staining was performed after ABCA1 was knocked down. c Total cholesterol contents after ABCA1 knockdown. d Membrane cholesterol levels were measured after ABCA1 knockdown. e Protein expression of Vimentin in HN6 and CAL27 cells treated with cholesterol and MβCD. f Effects of siABCA1 and cholesterol on the expression of Vimentin protein in HN6 and CAL27 cells. Statistical significance was assessed with Student’s t -test. * P < 0.05, ** P < 0.01, ** *P < 0.00 1

Article Snippet: The lysates were then incubated overnight at 4 °C with either an anti-ABCA1 antibody (DF8233, Affinity Biosciences) or IgG (30000-0-AP, Proteintech) with constant rotation.

Techniques: Membrane, Over Expression, Staining, Knockdown, Expressing

Journal: International Journal of Oral Science

Article Title: Antisense oligonucleotide targeting TARDBP-EGFR splicing axis inhibits progression of oral squamous cell carcinoma through ABCA1-regulated cholesterol efflux

doi: 10.1038/s41368-025-00402-7

Figure Lengend Snippet: Schematic illustration of the function and mechanism of the rs737540 in OSCC risk (By Figdraw). Compared with the rs737540-C allele, the risk rs737540-T allele promotes the inclusion of exon 4 of EGFR mediated by TARDBP to increase the expression of the full-length EGFR isoform ( EGFR-001 ) and decrease the expression level of the truncated EGFR isoform ( EGFR-004 ). Compared with the EGFR-004 subtype without exon 4, EGFR-001 was more able to inhibit ABCA1 ubiquitination and degradation by inhibiting EGFR phosphorylation, thus promoting OSCC cell proliferation, which provided functional evidence supporting our findings in a large population that rs737540-T contributes to increased OSCC risk. Meanwhile, ABCA1 can affect membrane fluidity by regulating membrane cholesterol levels. In addition, ASO targeting rs737540-T showed a significant tumor suppressor effect. The combination of ASO and Cetuximab shows broad prospects

Article Snippet: The lysates were then incubated overnight at 4 °C with either an anti-ABCA1 antibody (DF8233, Affinity Biosciences) or IgG (30000-0-AP, Proteintech) with constant rotation.

Techniques: Expressing, Ubiquitin Proteomics, Phospho-proteomics, Functional Assay, Membrane